ABSTRACT
BACKGROUND: Postoperative cholangitis is a common but severe complication after Kasai portoenterostomy for biliary atresia (BA). This study aimed to identify its prognostic factors. METHODS: Two sets of liver paraffin-embedded tissue samples were collected from BA patients who received Kasai portoenterostomy (n = 25 and n = 31, respectively). Patients were divided into non-cholangitis and cholangitis groups. The infiltration of CD4+, CD8+, CD45RO+, CD68+ cells and expression of Beclin1 were quantitatively evaluated in immunohistochemical analysis. RESULTS: Cholangitis group had a significantly lower CD8+ T cell infiltration but a higher CD45RO+ cell infiltration, and a lower Beclin1 level than non-cholangitis group (all P < 0.01). Multivariate logistic regression analysis indicated that infiltration of CD8+ cells (odds ratio [OR], 0.112; 95% confidence interval [CI], 0.022–0.577) and CD45RO+ cells (OR, 3.88; 95% CI, 1.37–11.03), and Beclin1 level (OR, 0.088; 95% CI, 0.018–0.452) were independent influence factors for early postoperative cholangitis. Receiver operating characteristic (ROC) analysis showed that area under ROC curve (AUROC) values for CD8+ cells, CD45RO+ cells and Beclin1 were 0.857, 0.738 and 0.900, respectively. CONCLUSION: Our findings demonstrated the CD8+ cells, CD45RO+ cells and Beclin1 level possessed the prognostic value for early postoperative cholangitis following Kasai operation, which may be helpful to develop new prevention and treatment strategies for postoperative cholangitis.
Subject(s)
Humans , Biliary Atresia , Cholangitis , Liver , Logistic Models , ROC Curve , T-LymphocytesABSTRACT
objective: To observe the effectiveness of vertical easy ball attachments (VEBA) in removable partial denture with metal base. Methods: Removable partial denture was retained with one or two sets of VEBA attached to full metal crown or porcelain fused to metal crown in 12 patients and 6～24 month clinical follow up was carried out. Results: All removable partial dentures retained with VEBA had good retention, stabilization, function and esthetic outcome, all the patients were greatly satisfied. Conclusion: VEBA has strong retentive force and protects abutments, it is a promising retainer in removable partial denture.